ASTM D4455-1985(2002) 按荧光显微镜计算程序进行水生菌计数的试验方法
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【英文标准名称】:StandardTestMethodforEnumerationofAquaticBacteriabyEpifluorescenceMicroscopyCountingProcedure
【原文标准名称】:按荧光显微镜计算程序进行水生菌计数的试验方法
【标准号】:ASTMD4455-1985(2002)
【标准状态】:现行
【国别】:
【发布日期】:1985
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:水;计算系统;试验;细菌纲;计数
【英文主题词】:aquaticbacteria;epifluorescence;epifluorescencemicroscopycountingprocedure
【摘要】:Bacterialpopulations,aspartofthemicrobialcommunityinaquaticsystemsareactivelyinvolvedinnutrientcycling.Thesignificanceofthesepopulationsisoftendifficulttoascertainbecauseofthepresenceofmanyphysiologicaltypes.However,measurementofbacterialdensitiesisusuallythefirststepintryingtoestablishanyrelationshipthatmightexistbetweenbacteriaandotherbiochemicalprocesses.4Acridine-orangeepifluorescencedirect-countingprocedurecannotdifferentiatebetweenviableandnonviablecells.Thisprocedurecannotbeusedtoconvertdirectlythenumberstototalcarbonbiomassbecauseofthenaturalvariationsinbacterialcellsize.Theacridine-orangeepifluorescencedirect-microscopiccountisbothquantitativeandprecise.Thisprocedureisidealforenumeratingbothpelagicandepibenthicbacteriainallfreshwaterandmarineenvironments.5Theprocesscanbeemployedinsurveyactivitiestocharacterizethebacteriologicaldensitiesofenvironmentalwaters.Theprocedurecanalsobeusedtoestimatebacterialdensitiesincoolingtowerwaters,processwaters,andwatersassociatedwithoildrillingwells.1.1Thistestmethoddescribesaprocedurefordetectionandenumerationofaquaticbacteriabytheuseofanacridine-orangeepifluorescencedirect-microscopiccountingprocedure.Itisapplicabletoenvironmentalwaters.1.2Certaintypesofdebrisandothermicroorganismsmayfluoresceinacridineorange-stainedsmears.1.3Thetestmethodrequiresatrainedmicrobiologistortechnicianwhoiscapableofdistinguishingbacteriafromotherfluorescingbodiesonthebasisofmorphologywhenviewedathighermagnifications.1.4Useofbrightlightpermitsdifferentiationofsinglebacteriawherereducedformazanisdepositedatthepolarends.1.5Approximately1014cells/mLarerequiredfordetectionbythistestmethod.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_20(MICROBIOLOGYOFWATER)
【页数】:3P.;A4
【正文语种】:
【原文标准名称】:按荧光显微镜计算程序进行水生菌计数的试验方法
【标准号】:ASTMD4455-1985(2002)
【标准状态】:现行
【国别】:
【发布日期】:1985
【实施或试行日期】:
【发布单位】:美国材料与试验协会(US-ASTM)
【起草单位】:D19.24
【标准类型】:(TestMethod)
【标准水平】:()
【中文主题词】:水;计算系统;试验;细菌纲;计数
【英文主题词】:aquaticbacteria;epifluorescence;epifluorescencemicroscopycountingprocedure
【摘要】:Bacterialpopulations,aspartofthemicrobialcommunityinaquaticsystemsareactivelyinvolvedinnutrientcycling.Thesignificanceofthesepopulationsisoftendifficulttoascertainbecauseofthepresenceofmanyphysiologicaltypes.However,measurementofbacterialdensitiesisusuallythefirststepintryingtoestablishanyrelationshipthatmightexistbetweenbacteriaandotherbiochemicalprocesses.4Acridine-orangeepifluorescencedirect-countingprocedurecannotdifferentiatebetweenviableandnonviablecells.Thisprocedurecannotbeusedtoconvertdirectlythenumberstototalcarbonbiomassbecauseofthenaturalvariationsinbacterialcellsize.Theacridine-orangeepifluorescencedirect-microscopiccountisbothquantitativeandprecise.Thisprocedureisidealforenumeratingbothpelagicandepibenthicbacteriainallfreshwaterandmarineenvironments.5Theprocesscanbeemployedinsurveyactivitiestocharacterizethebacteriologicaldensitiesofenvironmentalwaters.Theprocedurecanalsobeusedtoestimatebacterialdensitiesincoolingtowerwaters,processwaters,andwatersassociatedwithoildrillingwells.1.1Thistestmethoddescribesaprocedurefordetectionandenumerationofaquaticbacteriabytheuseofanacridine-orangeepifluorescencedirect-microscopiccountingprocedure.Itisapplicabletoenvironmentalwaters.1.2Certaintypesofdebrisandothermicroorganismsmayfluoresceinacridineorange-stainedsmears.1.3Thetestmethodrequiresatrainedmicrobiologistortechnicianwhoiscapableofdistinguishingbacteriafromotherfluorescingbodiesonthebasisofmorphologywhenviewedathighermagnifications.1.4Useofbrightlightpermitsdifferentiationofsinglebacteriawherereducedformazanisdepositedatthepolarends.1.5Approximately1014cells/mLarerequiredfordetectionbythistestmethod.1.6Thisstandarddoesnotpurporttoaddressallofthesafetyconcerns,ifany,associatedwithitsuse.Itistheresponsibilityoftheuserofthisstandardtoestablishappropriatesafetyandhealthpracticesanddeterminetheapplicabilityofregulatorylimitationspriortouse.
【中国标准分类号】:Z16
【国际标准分类号】:07_100_20(MICROBIOLOGYOFWATER)
【页数】:3P.;A4
【正文语种】:
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